Students studying M.Sc/ M.Phil/
PhD/ B.Tech/M.Tech Belonging to various Life Science Braches can do Major and Minor Projects at Derex Labs.
List of Projects
ENVIRONMENTAL BIOTECHNOLOGY
Isolation, identification
and molecular characterization of microbes from effluents of textile, leather, paper and other industries.
Isolation
and characterization of microbes involved in degradation of
pesticides
and insecticides.
Isolation
of microbes from uranium or iron contaminated soil.
Comparative and
mutation studies on biodegradation.
Biodegradation
of thermal effluents.
Biodegradation
of PHA.
Biodegradation
of Hydrocarbons.
Biodegradation
of azodyes.
Study on biodegradable
enzymes like peroxides, laccase, azo-reductase...etc.
Biodegradation
of lignin compounds.
Bioleaching (studies
on heavy metals).
Characterization
of phytoflora near industries.
Effect
of pesticides/insecticides on bio fertilizers.
Xenobiotics
degradation.
ENZYME BIOTECHNOLOGY
Isolation, purification, characterization
of sera peptidase/ heparins/ alkaline phosphatase/ glucose Oxidase/ glucoamylase/ glucanase and other enzymes from microbial
and plant sources.
Isolation and purification of recalcitrant
degrading enzymes like magnesium peroxides, laccase, azoreductase etc.
Isolation, purification and kinetic
studies on enzymes from animal sources (collagenase, alkaline phosphatase, lysozyme, etc).
Isolation, purification
and characterization of luctoperoxidase.
Isolation, purification
and characterization of microbial rennet enzyme and comparative analysis with animal rennet enzyme and bacteria from different
types of cheese production.
Isolation and purification of pululanase/
inulinase from microbes.
Comparative study on the production
of lipase/alpha amylase/serine protease/polymerase/tyrosine's/diastase from microbes.
Strain improvement by mutational
studies for the production and characterization of enzymes.
Production of
enzymes showing anti-cancer activity from microbes.
Production, purification
and characterization of Agarase.
Production, purification
and characterization of Asparaginase.
Production, purification
and characterization of Chitinase.
Production, purification
and characterization of Galactosidase.
Production, purification
and characterization of Isoamylase.
Production, purification
and characterization of Laccase.
Production, purification
and characterization of Lactase
Production, purification
and characterization of Pectinase.
Production, purification
and characterization of Phytase.
Production, purification
and characterization of Cucumsin.
Production, purification
and characterization of Lipoxidase.
Production, purification
and characterization of Ficin.
Production, purification
and characterization of Tyrosinase.
Production, purification
and characterization of Urease.
Production, purification
and characterization of Xylanase.
Production, purification
and characterization of Naringinase.
Production, purification
and characterization of Nitrate reductase.
Production, purification
and characterization of Lysozyme.
Production, purification,
characterization and comparison of Alpha & Bita- Amylase.
Extraction and purification of bromolien/papain
/polyphenol Oxidase/ peroxides.
Extraction, purification
and characterization of Bromelain and comparative study of Bioevents between Immobilized Biocatalyst versus its soluble counter
parts.
Extraction and
purification of Papain from Calica Papaya.
MOLECULAR BIOLOGY
Basic Molecular Biology:
DNA isolation of plasmid DNA
DNA
isolation from plant DNA
Genomic DNA
isolation
Bacterial Transformation
Restriction
Ligation
PCR
Quantitative estimation of DNA
Purification of DNA
SDS-PAGE, a molecular separation
technique
Gel Electrophoresis and applicability in Industries
Advance techniques
An introduction to Recombinant DNA
Technology
Isolation of DNA
from fruit
Isolation and purification of chromosomal
DNA from yeast / plant / animal
Isolation, purification and testing
of Antimicrobial activity of Beta-Lactam Antibiotics from Fungus and Detection of pcbC
gene using PCR.
Isolation and purification of total
DNA from prokaryote
Isolation of Plasmid DNA
from prokaryotes
Instrumentation in Recombinant DNA
Technology
Quantification of DNA
Gel Electrophoresis Techniques (PAGE
& SDS-PAGE) and applicability in Industries
Agarose Gel Electrophoresis
U.V. Transillumination and applicability
in Biotech industries
Blotting techniques in Biotechnology
Southern Blotting and its application
spectrum
PCR Basics
Primer screening
Amplification of gene of interest
Preparation of Competent cells
Transformation of Recombinant DNA
into host cells
Screening of Competent cells
Sequencing of DNA*(optional)
Protein Purification
Gene detection & Characterization
of Biodegrading microbs (Styrene, polyethylene and thermocol).
Comparative Transformation studies
of various plasmids DNA’s in E. coli.
Assesment of cry-gene of BT toxin.
PCR analysis of Microorganisms.
Comparison of FAME GC method against conventional PCR analysis in detection of microbes.
Restriction mapping of medicinal
plants and pathogenic organism.
Molecular characterization –RFLP,
RAPD, AFLP, SSR, CAPs, iSSR.
Identification of varietals purity
using markers. (MAS)
Gene cloning and expression and identification
by western blotting (chitins, GST, GFP).
Gene identification and characterization
by using PCR.
Expression, Gene regulation and molecular
characterization of protein molecules.
Genetic and protein characterization
of microbes (e.g. - Pseudomonas sps, E.coli, etc.).
Agro bacterium mediated gene transformation,
expression and characterization.
Detection of LuxG from Vibrio harveyi,
its cloning and expression in E-coli & its insilico analysis.
Cloning and Expression of GFP gene
in Bacteria and Yeast.
Production of bacitracin, and Cloning
and expression of BcrR gene.
Industrial biotechnology
U.V.
Transillumination and the applicability in Biotech industries.
Evaluation
of industrial applications of extracted enzymes.
Down
stream process.
Isolation,
purification and characterization of Cellulase.
Isolations
and Identification of anaerobic from industrial waste water.
Isolation of microorganism
from dairy product.
Isolation, purification
and characterization of Bacitracin from Bacillus Subtilis and Bacillus licheniformis.
Isolation, production,
purification and antimicrobial assay of polymyxin from Bacillus subtilis
Isolation, purification
and biochemical characterization of Antibiotics from Microorganisms
Production of
antibiotics from different fungal source and its comparative effects
on different bacterial pathogens.
Production
of Protease / Amylase /Cellulose enzyme.
Production of
sauerkraut, cheese and other diary products using different substrate from microorganisms.
Production of
different enzymes using bacterial and fungal species.
Production and
purification of lactic acid.
Production and
purification of aconitic acid.
Production and
purification of acetic acid
Production, purification
and characterization of Polypeptide Antibiotics "Polymyxin B & Cerexin A" From Bacillus species
Production, purification
of Streptomycin and Erythromycin from Streptomyces strain.
Synthesis and
purification of different amino acid from microorganisms and its characterization.
Comparative analysis
of lactic acid production from different species.
Comparatively
study of Penicillin production from wild and Mutant strain of penicillium Chrysogenum and Aspergilus nidulans.
Enhancement of
citric acid production from fungal species by using additives.
Enhancement
of citric acid production from fungal species by using additives
Comparative
analysis of lactic acid production from different species
Food Beverage
and environment assessment.
Immunology
immunoprecopitation
a. Ouchterlony’s immuno diffusion technique
b. Counter current immuno electrophoresis
Immunoglobulin purification and characterization
Differtial (identification of cell types) and total leukocytes counts of blood
Isolation and viability determination of lymphocytes from peripheral blood
Lymphocyte proliferation with mitogen and migration with capillary tubes.
Identification of cell types by receptors immunofluroscence
Raising of antibodies in animals – polyclonal antibodies (against plant or animal pathogens)
Antibiotic assay
Rocket immunelectrophoresis
Raising polyclonal
antibodies against plant pathogens and detection using ELISA.
Raising polyclonal
antibodies against antigens from pathogenic microorganisms.
Purification of
antibodies and characterization
Microbial biotechnology
Antimicrobial
studies of plants secondary metabolites.
Screening for
the production of antimicrobial compounds from fungi.
Screening for
the production of antimicrobial compounds from Actinomycetes.
Isolation, cultivation
and characterization of extremophillic microorganisms.
Isolation and
characterization of Actinomycetes from different sources.
Isolation and
characterization of antimicrobial compund from Bacillus coagulans.
Isolation of Lactic
acid producing bacteria and checking inhibitory effect of lactic acid in pathogenic microorganisms.
Isolation of different
strain of Lactobacillus acidophilus , examination for production of antagonism and inhibitory activity against spoilage microoranism.
Isolation of luctoperoxidase,
lactoferrin and lactoferrcin from milk.
Isolation, purification
and characterization of Cellulase.
Streptomyces-
derived antimicrobial compound and method of using same against antibiotic resistant bacteria .
Study of different
antimicrobial compunds.
Antimicrobial
activity of of medicinaly important plant.
Antimicrobial
activity of Bacillus subtilis, Bacillus pumilus and Bacillus lecheniformis.
Essential oil
tested for its antimicrobial acitivity.
Naturally occuring
antimicrobial compounds from plant.
Antimicrobial
activity of Pseudomonas fluorescens.
Classification
of Bacteria isolated from pozol , exhibiting antimimicrobail activity against several gram positive and gram negative bacteria,
yeast and mold.
Identification
and antimicrobial activity of actinomycetes from soil .
Study of antimicrobial
activity of Lactobacillus sps.
To check the efficency
of antimicrobail compounds.
Natural antioxidant
constituents from selected aromatic plant and their antimicrobial activity against selected pathogenic microorganism.
Effect of physical
method for pathogne.
Bacteriocin from
gram positive and gram negative organism and their complete mechanism.
Antimicrobial
activities of fruits.
Antimicrobial
activity of yeast.
Antimicrobial
activity of lysozyme.
Antimicrobial
activity of egg.
Antimicrobial
activity of essential oil, saponins, alkoloid, thiosulfinate,glucosinolate, flavonoid, galangal, vanill etc.
Comparative analysis
of Patulin produced from Penicillum expansum & Aspergillus flavus
Comparative study
of Aflatoxin & Aspergillic acid production from Aspergillus flavus ana Aspergillus niger
by Submerged Fermentation
Comparative analysis
of Siderophore production in Pseudomonas fluorescens.
Production and
Purification of vibriocin, subtilin, colicin and casein.
Production by
Bacillus pumilus (MSH) of an antifungal compound that is active against Mucoraceae and Aspergillus species.
Production of
antibiotics from different fungal source and its comparative effects on different bacterial pathogens.
Production, purification
and characterization of Acidolin from Lactobacillus Acidophilus
Production and
antimicrobial activity of pediocin.
Production and
antimicrobial activity of Reuterin.
Production of
Biofertilizers
MICROBIOLOGY
Bacterial
Growth curve
Isolation of microorganisms from natural sources
Isolation and
identification of harmful pathogens from commercial cheese (by conventional methods/by Fame analysis).
Isolation and
identification of microorganism from hard surfaces (computer key board, ATM screen, phone receivers, public phone booth, Lift
operating Button etc).
Isolation and identification of microorganism found in flowers.(Jasmin,rose and hibiscus.)
Isolation and identification of TT(Clostridium
tetanii).
Isolation and Identification of harmful pathogens from soil.
Isolation and identification of fermentative microbes from fermented products.
Medical biotechnology
Isolation of different
pathogenic bacteria from clinical samples and comparative inhibition by commercially available antibiotics.
Plant Biotechnology:
Isolation
and characterization of plant disease causing bacteria and disease control
Induction of Crown
gall tumors to improve the production of Secondary Metabolites in Artemisia absinthium.
Extraction, production
and purification of plant secondary Metabolites and testing for its Antimicrobial Activity.
ANTIMICROBIAL EFFECT ON NATURAL SOURCES (MICROBIOLOGY/BIOTECHNOLOGY
Screening for the production of antimicrobial compounds from fungi.
Production of antibiotics from different fungal source and its comparative effects on different bacterial
pathogens.
Isolation and characterization of Actinomycetes from soil.
Streptomyces- derived antimicrobial compound and method of using same against antibiotic resistant bacteria.
Effect of different disinfectants on microbes.
Antimicrobial activity of Clove, Garlic,Tulasi and Neem Leaves .
Antimicrobial activity of Bacillus subtilis, Salmonella, pseudomonas
Antimicrobial property of Camphor, Turmeric and sandal wood.
Antimicrobial activities of Citraus fruits.
Antimicrobial activity of egg.
Antimicrobial activity of essential oil, saponins, etc.
Antimicrobial activity of Lactobacillus sps.
To check the efficiency of antimicrobial compounds.
Effect of physical & chemical stress method on microorganisms.
Isolation of microorganisms
from natural sources.
Isolation of antibiotic producing microorganisms.(Actinomycetes)
Determination of MIC of antibiotics.
EXPERIMENTS ON FAME ANALYSIS
Identification
of fungal source and its comparative effects on different bacterial pathogens
Identification of bacteria from blood culture by Fatty Acid Methyle Ester Analysis Through Gas Chromatography.
Identification of microbes by fatty acid analysis
Isolation and identification of microorganisms present in street food by phenotypic methods especially by Fatty Acid Methyle Ester Analysis Through Gas Chromatography.
Isolation and ident ification of microorganism from Soil by Fatty Acid Methyle Ester Analysis Through Gas Chromatography.
Isolation
of microorganism wth lipolytic activity from Probiotics.
Isolation
and identification of microorganism from spoiled food.
Isolation
of different pathogenic bacteria from clinical samples and comparative inhibition by commercially available antibiotics
Isolation and characterization of microbes involved in degradation of
pesticides and insecticides
Isolation and identification of microorganisms capable of Styrene Degradation.
Enumeration
of Microorganism with proteolytic activity from Probiotics.
Analysis
and identification of lactic acid in Bacillus sporogenes for efficacy in market
sample.
Effect
of preservatives on microorganism in Preserve foods.( Pickles, jams ,sauces, juices)
Quantitative
analysis of Virginity of olive oil samples .
Bacterial identification by Fatty Acid Methyle Ester Analysis Through
Gas Chromatography in commercially manufactured foods.
Fatty acid analysis of soil sample.
Phospholipids fatty acid (PLFA) analysis from soil sample
Classification of microorganism by analysis of lipids.
Role of gas chromatography in clinical Microbiology lab.
Gas Chromatography in identification of microorganisms
and diagnosis of infectious diseases.
Use of MIDI-FAME technique to characterize ground water communities
BIOINFORMATICS
Basic Bioinformatics-15 days
Introduction to Bioinformatics and overview of Biological Research
Biological databases and database searching
Data Mining and Sequence Retrieval
Motif & Domain assignment
Proteomics
Protein threading and interaction
Pair wise and Multiple Sequence Alignment
Sequence Analysis (BLAST, FASTA, CLUSTAL W)
Phylogenetic analysis
Rational Drug designing
Introduction to chemioinformatics and Drug Development
Chemical and Drug Database
Proteomics
Sequence analysis of Receptor molecule
Structural Bioinformatics in Drug Development
Small molecule hits
Designing chemical structures
Pathways studies and Drug Targeting
Lead design with Geometry optimization and Energy Minimization
Chemical reaction indexing
Pharmacophore analysis
Docking
Drug Designing -45 days
Introduction to chemioinformatics and Drug Development
Drug databases and Data Mining
Structural Bioinformatics in drug discovery
Force Fields and Minimizers Concept
Geometry optimization and Energy minimization
Docking
Relevant Softwares
Bioinformatics in Protein Modeling, Drug
Designing and Immunoinformatics-90 days
Introduction to Bioinformatics and overview of Biological Research
Biological databases and database searching
Data Mining and Sequence Retrieval
Motif & Domain assignment
Proteomics
Protein threading and interaction
Pair wise and Multiple Sequence Alignment
Sequence Analysis (BLAST, FASTA, CLUSTAL W)
Phylogenetic analysis
Ab initio structure prediction
Visualization tools
Homology Modeling
Introduction to chemioinformatics and Drug Development
Chemical and Drug Database
Structural Bioinformatics in Drug Development
Small molecule hits
Designing Chemical Structures
Pathways studies and Drug Targeting
Lead design with Geometry optimization and Energy Minimization
Computer simulations
Chemical reaction indexing
Pharmacophore analysis
Docking
Chemical Patents
FDA in drug trials
Introduction to Immunoinformatics and Computational Vaccinology
Accessing genome agencies
Vaccine database and Data mining
T-Cells development
Antigen processing and presentation
Reverse Vaccinology and Vaccine screening
MHC binding
Organ Transplant and Auto Immune diseases
Epitope prediction and Servers
In Silico approach towards AIDS Vaccine and Avian Influenza Virus
Vaccine design
Relevant Tools and Soft wares
AGRICULTURE
Response
in germination and seedling growth of plant under salt condition.
Isolation
of organisms present in fresh leaf & infected leaf
Isolation
and identification of selective organisms present in soil & carry out its CFU count
Isolation
and identification of organisms present in industrial waste water
Determination
& comparison of lipid biomass in soil & rhizobium by FAME analysis
Testing
of soil and analyzing different parameters like pH, mineral content and soil fertility
Checking
the effect of heavy metals on plant growth.
