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HPLC Training - Derex Labs

Method development Training
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Hands on Method Development Training

I.      Method Development:

 

a.       Introduction: Purpose and Scope of analytical method development. Its Advantages and applications

b.       Setup the method development goals

c.       Understand the chemistry of analytes

                                                               i.      pH

                                                             ii.      Solubility

                                                            iii.      Melting point

                                                           iv.      Polarity

                                                             v.      Basic structure

                                                           vi.      Synthesis  process

                                                          vii.      Possible degradants/ Impurities

d.      Literature collection or search for compendial methods

e.       Select a suitable chromatography method  like HPLC or GC

f.        Started with Primary conditions

g.       Optimization of

                                                               i.      Column

1.      Choice of column

2.      Polarity

3.      Dimensions

4.      Reproducibility

5.      Bad peak shape

6.      temperature

                                                             ii.      Mobile phase

1.      Reverse Phase or Normal Phase

2.      Gradient or Isocratic

3.      Solvent Strength

4.      Buffer Capacity

5.      Buffer UV absorption

6.      Preferred buffers

7.      Effect of pH and Ion pairing

                                                            iii.      Selecting or optimize Analyte  concentration

                                                           iv.      System suitability

1.      Resolution

2.      Peak Tailing

3.      Column Theoretical plates and

4.      Relative standard deviation

 

                                                             v.      Detection Sensitivity and Selectivity

1.      Types of Detectors

2.      General Considerations

3.      Selecting suitable detector

4.      Choice of Wave lengths

5.      Optimize the S/N ratio

6.      Optimize the maximum S/N  for Assay and other peaks

 

                                                           vi.      Completing the method development

1.      Checking of repeatability

2.      Analyzing available samples (Different lots)

3.      Perform the preliminary validation

a.       Precision

b.      Linearity

c.       Accuracy

d.      Robustness

 

II.    Trouble Shooting LC and GC Systems:

LC System: During the method development, these are the common problems arise

A.     Pressure (High or Low)

B.     Leaks

C.     Retention time problems

D.     Inaccurate Results

E.      Base line noise instability

F.      Poor Peak Shapes

G.     Extra Peaks

H.     Injector problems

GC System:

A.     No Peaks

B.     Poor sensitivity with normal retention

C.     Negative peaks

D.     Base line drift

E.      Constant base line drift in one direction

F.      High background signal

 

III. Prevention of Problems:

A.                 Selecting initial Components

B.                 Routine preventive maintenance

C.                 Early replacement of critical parts

D.                 Record Keeping

For details and training Schedule contact us on 9949974890/9246882890