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Academic Projects Biotech/Life Sciences

 

 

 

Students studying M.Sc/ M.Phil/ PhD/ B.Tech/M.Tech Belonging to various Life Science Braches can do Major and Minor Projects at Derex Labs.

 

                                                                                              

List of Projects

 

 

 

ENVIRONMENTAL BIOTECHNOLOGY

 

Isolation, identification and molecular characterization of microbes from effluents of textile, leather, paper and other industries.

Isolation and characterization of microbes involved in degradation of  

pesticides and insecticides.

Isolation of microbes from uranium or iron contaminated soil.

Comparative and mutation studies on biodegradation.

Biodegradation of thermal effluents.

Biodegradation of PHA.

Biodegradation of Hydrocarbons.

Biodegradation of azodyes.

Study on biodegradable enzymes like peroxides, laccase, azo-reductase...etc.

Biodegradation of lignin compounds.

Bioleaching (studies on heavy metals).

Characterization of phytoflora near industries.

Effect of pesticides/insecticides on bio fertilizers.

Xenobiotics degradation.

 

 

 

 

 

 

 

 

 

 

 

ENZYME BIOTECHNOLOGY

 

Isolation, purification, characterization of sera peptidase/ heparins/ alkaline phosphatase/ glucose Oxidase/ glucoamylase/ glucanase and other enzymes from microbial and plant sources.

 

Isolation and purification of recalcitrant degrading enzymes like magnesium peroxides, laccase, azoreductase etc.

 

Isolation, purification and kinetic studies on enzymes from animal sources (collagenase, alkaline phosphatase, lysozyme, etc).

 

Isolation, purification and characterization of luctoperoxidase.

Isolation, purification and characterization of microbial rennet enzyme and comparative analysis with animal rennet enzyme and bacteria from different types of cheese production.

Isolation and purification of pululanase/ inulinase from microbes.

 

Comparative study on the production of lipase/alpha amylase/serine protease/polymerase/tyrosine's/diastase from microbes.

 

Strain improvement by mutational studies for the production and characterization of enzymes.

 

Production of enzymes showing anti-cancer activity from microbes.

Production, purification and characterization of Agarase.

Production, purification and characterization of Asparaginase.

Production, purification and characterization of Chitinase.

Production, purification and characterization of Galactosidase.

Production, purification and characterization of Isoamylase.

Production, purification and characterization of Laccase.

Production, purification and characterization of Lactase

 

 

 

 

Production, purification and characterization of Pectinase.

Production, purification and characterization of Phytase.

Production, purification and characterization of Cucumsin.

Production, purification and characterization of Lipoxidase.

Production, purification and characterization of Ficin.

Production, purification and characterization of Tyrosinase.

Production, purification and characterization of Urease.

Production, purification and characterization of Xylanase.

Production, purification and characterization of Naringinase.

Production, purification and characterization of Nitrate reductase.

Production, purification and characterization of Lysozyme.

Production, purification, characterization and comparison of  Alpha & Bita- Amylase.

Extraction and purification of bromolien/papain /polyphenol Oxidase/ peroxides.

Extraction, purification and characterization of Bromelain and comparative study of Bioevents between Immobilized Biocatalyst versus its soluble counter parts.

Extraction and purification of Papain from Calica Papaya.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

MOLECULAR BIOLOGY

 

 

Basic Molecular Biology:

 

DNA isolation of plasmid DNA

DNA isolation from plant DNA

Genomic DNA isolation

Bacterial Transformation

Restriction

Ligation

PCR

Quantitative estimation of DNA

Purification of DNA

SDS-PAGE, a molecular separation technique

Gel Electrophoresis and applicability in Industries

 

Advance techniques

 

An introduction to Recombinant DNA Technology

Isolation of DNA from fruit

Isolation and purification of chromosomal DNA from yeast / plant / animal

Isolation, purification and testing of Antimicrobial activity of Beta-Lactam Antibiotics from Fungus and Detection of  pcbC gene using PCR.

Isolation and purification of total DNA from prokaryote

Isolation of Plasmid DNA from prokaryotes

Instrumentation in Recombinant DNA Technology

Quantification of DNA

Gel Electrophoresis Techniques (PAGE & SDS-PAGE) and applicability in Industries

Agarose Gel Electrophoresis

U.V. Transillumination and applicability in Biotech industries

Blotting techniques in Biotechnology

Southern Blotting and its application spectrum

PCR Basics

 

 

 

 

 

Primer screening

Amplification of gene of interest

Preparation of Competent cells

Transformation of Recombinant DNA into host cells

Screening of Competent cells

Sequencing of DNA*(optional)

Protein Purification

Gene detection & Characterization of Biodegrading microbs (Styrene, polyethylene and thermocol).

Comparative Transformation studies of various plasmids DNA’s in E. coli. 

Assesment of cry-gene of BT toxin. 

PCR analysis of Microorganisms.

  Comparison of FAME GC method against conventional PCR analysis in detection of microbes.

Restriction mapping of medicinal plants and pathogenic organism.

 

Molecular characterization –RFLP, RAPD, AFLP, SSR, CAPs, iSSR.

 

Identification of varietals purity using markers. (MAS)

 

Gene cloning and expression and identification by western blotting (chitins, GST, GFP).

 

Gene identification and characterization by using PCR.

 

Expression, Gene regulation and molecular characterization of protein molecules.

 

Genetic and protein characterization of microbes (e.g. - Pseudomonas sps, E.coli, etc.).

 

Agro bacterium mediated gene transformation, expression and characterization.

 

Detection of LuxG from Vibrio harveyi, its cloning and expression in E-coli & its insilico analysis.

Cloning and Expression of GFP gene in Bacteria and Yeast.

Production of bacitracin, and Cloning and expression of BcrR gene.

 

 

 

 

 

 

 

 

 

 

 

 

Industrial biotechnology

 

U.V. Transillumination and the applicability in Biotech industries.

 

Evaluation of industrial applications of extracted enzymes.

Down stream process.

Isolation, purification and characterization of Cellulase.

Isolations and Identification of anaerobic from industrial waste water.

Isolation of microorganism from dairy product.

Isolation, purification and characterization of Bacitracin from Bacillus Subtilis and Bacillus licheniformis.

Isolation, production, purification and antimicrobial assay of polymyxin from Bacillus subtilis

Isolation, purification and biochemical characterization of Antibiotics from Microorganisms

Production of antibiotics from different fungal source and its comparative    effects on different bacterial pathogens.

Production of Protease / Amylase /Cellulose enzyme.

Production of sauerkraut, cheese and other diary products using different substrate from microorganisms.

Production of different enzymes using bacterial and fungal species.

Production and purification of lactic acid.

Production and purification of aconitic acid.

Production and purification of acetic acid

Production, purification and characterization of Polypeptide Antibiotics "Polymyxin B & Cerexin A" From Bacillus species

 

 

 

 

 

 

 

Production, purification of Streptomycin and Erythromycin from Streptomyces strain.

Synthesis and purification of different amino acid from microorganisms and its characterization.

Comparative analysis of lactic acid production from different species.

Comparatively study of Penicillin production from wild and Mutant strain of penicillium Chrysogenum and Aspergilus nidulans.

Enhancement of citric acid production from fungal species by using additives.

Enhancement of citric acid production from fungal species by using additives

Comparative analysis of lactic acid production from different species

Food Beverage and environment assessment.

 

Immunology

 

immunoprecopitation

a.       Ouchterlony’s immuno diffusion technique

b.      Counter current immuno electrophoresis

 

Immunoglobulin purification and characterization

Differtial (identification of cell types) and total leukocytes counts of blood

Isolation and viability determination of lymphocytes from peripheral blood

Lymphocyte proliferation with mitogen and migration with capillary tubes.

Identification of cell types by receptors immunofluroscence

Raising of antibodies in animals – polyclonal antibodies (against plant or animal pathogens)

Antibiotic assay

Rocket immunelectrophoresis

Raising polyclonal antibodies against plant pathogens and detection using    ELISA.

Raising polyclonal antibodies against antigens from pathogenic microorganisms.

Purification of antibodies and characterization  

 

 

 

 

 

 

 

 

 

 

 

 

Microbial biotechnology

 

Antimicrobial studies of plants secondary metabolites.

Screening for the production of antimicrobial compounds from fungi.

Screening for the production of antimicrobial compounds from Actinomycetes.

Isolation, cultivation and characterization of extremophillic microorganisms.

Isolation and characterization of Actinomycetes from different sources.

Isolation and characterization of antimicrobial compund from Bacillus coagulans.

Isolation of Lactic acid producing bacteria and checking inhibitory effect of lactic acid in pathogenic microorganisms.

Isolation of different strain of Lactobacillus acidophilus , examination for production of antagonism and inhibitory activity against spoilage microoranism.

Isolation of luctoperoxidase, lactoferrin and lactoferrcin from milk.

Isolation, purification and characterization of Cellulase.

Streptomyces- derived antimicrobial compound and method of using same against antibiotic resistant bacteria .

Study of different antimicrobial compunds.

Antimicrobial activity of of medicinaly important plant.

Antimicrobial activity of Bacillus subtilis, Bacillus pumilus and Bacillus lecheniformis.

Essential oil tested for its antimicrobial acitivity.

Naturally occuring antimicrobial compounds from plant.

Antimicrobial activity of Pseudomonas fluorescens.

Classification of Bacteria isolated from pozol , exhibiting antimimicrobail activity against several gram positive and gram negative bacteria, yeast and mold.

Identification and antimicrobial activity of actinomycetes from soil .

 

 

 

 

 

 

 

Study of antimicrobial activity of Lactobacillus sps.

To check the efficency of antimicrobail compounds.

Natural antioxidant constituents from selected aromatic plant and their antimicrobial activity against selected pathogenic microorganism.

Effect of physical method for pathogne.

Bacteriocin from gram positive and gram negative organism and their complete mechanism.

Antimicrobial activities of fruits.

Antimicrobial activity of yeast.

Antimicrobial activity of lysozyme.

Antimicrobial activity of egg.

Antimicrobial activity of essential oil, saponins, alkoloid, thiosulfinate,glucosinolate, flavonoid, galangal, vanill etc.

Comparative analysis of Patulin produced from Penicillum expansum & Aspergillus flavus

Comparative study of Aflatoxin & Aspergillic acid production from Aspergillus flavus ana Aspergillus niger by Submerged Fermentation

Comparative analysis of Siderophore production in Pseudomonas fluorescens.

Production and Purification of vibriocin, subtilin, colicin and casein.

Production by Bacillus pumilus (MSH) of an antifungal compound that is active against Mucoraceae and Aspergillus species.

Production of antibiotics from different fungal source and its comparative effects on different bacterial pathogens.

Production, purification and characterization of Acidolin from Lactobacillus Acidophilus

Production and antimicrobial activity of pediocin.

Production and antimicrobial activity of Reuterin.

Production of Biofertilizers

 

 

MICROBIOLOGY

 

Bacterial Growth curve

Isolation of microorganisms from natural sources

Isolation and identification of harmful pathogens from commercial cheese (by conventional methods/by Fame analysis).

Isolation and identification of microorganism from hard surfaces (computer key board, ATM screen, phone receivers, public phone booth, Lift operating Button etc).

Isolation and identification of microorganism found in flowers.(Jasmin,rose and hibiscus.)

Isolation and identification of TT(Clostridium tetanii).

Isolation and Identification of harmful pathogens from soil.

Isolation and identification of fermentative microbes from fermented products.

 

 

Medical biotechnology

 

Isolation of different pathogenic bacteria from clinical samples and comparative inhibition by commercially available antibiotics.

 

Plant Biotechnology:

 

Isolation and characterization of plant disease causing bacteria and disease control

Induction of Crown gall tumors to improve the production of Secondary Metabolites in Artemisia absinthium.

Extraction, production and purification of plant secondary Metabolites and testing for its Antimicrobial Activity.

 

 

 

 

 

 

 

 

 

ANTIMICROBIAL EFFECT ON NATURAL SOURCES (MICROBIOLOGY/BIOTECHNOLOGY

 

 

Screening for the production of antimicrobial compounds from fungi.

Production of antibiotics from different fungal source and its comparative effects on different bacterial pathogens.

Isolation and characterization of Actinomycetes from soil.

Streptomyces- derived antimicrobial compound and method of using same against antibiotic resistant bacteria.

Effect of different disinfectants on microbes.

Antimicrobial activity of Clove, Garlic,Tulasi and Neem Leaves .

Antimicrobial activity of Bacillus subtilis, Salmonella, pseudomonas

Antimicrobial property of Camphor, Turmeric and sandal wood.

Antimicrobial activities of Citraus fruits.

Antimicrobial activity of egg.

Antimicrobial activity of essential oil, saponins,  etc.

Antimicrobial activity of Lactobacillus sps.

To check the efficiency of antimicrobial compounds.

Effect of physical & chemical stress method on microorganisms.

Isolation of microorganisms from natural sources.

Isolation of antibiotic producing microorganisms.(Actinomycetes)

Determination of MIC of antibiotics.

 

 

 

 

 

 

 

EXPERIMENTS ON FAME ANALYSIS

 

Identification of fungal source and its comparative effects on different bacterial pathogens

Identification of bacteria from blood culture by Fatty Acid Methyle Ester  Analysis Through Gas Chromatography.

Identification of microbes by fatty acid analysis

Isolation and identification of microorganisms present in street food by phenotypic methods especially  by Fatty Acid Methyle Ester  Analysis Through Gas Chromatography.

Isolation and ident ification of microorganism from Soil by Fatty Acid Methyle Ester  Analysis Through Gas Chromatography.

Isolation of microorganism wth lipolytic activity from Probiotics.

Isolation and identification of microorganism from spoiled food.

Isolation of different pathogenic bacteria from clinical samples and comparative inhibition by commercially available antibiotics

 

Isolation and characterization of microbes involved in degradation of  pesticides and insecticides

Isolation and identification of microorganisms capable of Styrene Degradation.

Enumeration of Microorganism with proteolytic activity from Probiotics.

 

Analysis and identification of lactic acid in Bacillus sporogenes for efficacy in market sample.

 

Effect of preservatives on microorganism in Preserve foods.( Pickles, jams ,sauces, juices)

 

Quantitative analysis of Virginity of olive oil samples .

 

Bacterial identification by Fatty Acid Methyle Ester  Analysis Through Gas Chromatography in commercially manufactured foods.

 

 

 

 

 

 

Fatty acid analysis of soil sample.

Phospholipids fatty acid (PLFA) analysis from soil sample

Classification of microorganism by analysis of lipids.

Role of gas chromatography in clinical Microbiology lab.

 Gas Chromatography in identification of microorganisms and diagnosis of infectious diseases.

Use of MIDI-FAME technique to characterize ground water communities

 

BIOINFORMATICS

 

Basic Bioinformatics-15 days

 

Introduction to Bioinformatics and overview of Biological Research

Biological databases and database searching

Data Mining and Sequence Retrieval

Motif & Domain assignment

Proteomics

Protein threading and interaction

Pair wise and Multiple Sequence Alignment

Sequence Analysis (BLAST, FASTA, CLUSTAL W)

Phylogenetic analysis

 

 

Rational Drug designing

 

Introduction to chemioinformatics and Drug Development

Chemical and Drug Database

Proteomics

Sequence analysis of Receptor molecule

Structural Bioinformatics in Drug Development

Small molecule hits

Designing chemical structures

Pathways studies and Drug Targeting

Lead design with Geometry optimization and Energy Minimization

Chemical reaction indexing

Pharmacophore analysis

Docking

 

 

Drug Designing -45 days

 

Introduction to chemioinformatics and Drug Development

Drug databases and Data Mining

Structural Bioinformatics in drug discovery

Force Fields and Minimizers Concept

Geometry optimization and Energy minimization

Docking

Relevant Softwares

 

 

Bioinformatics in Protein Modeling, Drug

Designing and Immunoinformatics-90 days

 

Introduction to Bioinformatics and overview of Biological Research

Biological databases and database searching

Data Mining and Sequence Retrieval

Motif & Domain assignment

Proteomics

Protein threading and interaction

Pair wise and Multiple Sequence Alignment

Sequence Analysis (BLAST, FASTA, CLUSTAL W)

Phylogenetic analysis

Ab initio structure prediction

Visualization tools

Homology Modeling

Introduction to chemioinformatics and Drug Development

Chemical and Drug Database

Structural Bioinformatics in Drug Development

Small molecule hits

Designing Chemical Structures

Pathways studies and Drug Targeting

Lead design with Geometry optimization and Energy Minimization

Computer simulations

Chemical reaction indexing

Pharmacophore analysis

Docking

Chemical Patents

FDA in drug trials

Introduction to Immunoinformatics and Computational Vaccinology

Accessing genome agencies

Vaccine database and Data mining

 

 

 

 

T-Cells development

Antigen processing and presentation

Reverse Vaccinology and Vaccine screening

MHC binding

Organ Transplant and Auto Immune diseases

Epitope prediction and Servers

In Silico approach towards AIDS Vaccine and Avian Influenza Virus

Vaccine design

Relevant Tools and Soft wares

 

 

AGRICULTURE

 

Response in germination and seedling growth of plant under salt condition.

Isolation of organisms present in fresh leaf & infected leaf

Isolation and identification of selective organisms present in soil & carry out its CFU count

Isolation and identification of organisms present in industrial waste water

Determination & comparison of lipid biomass in soil & rhizobium by FAME analysis

Testing of soil and analyzing different parameters like pH, mineral content and soil fertility

Checking the effect of heavy metals on plant growth.

 

 

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